排序方式: 共有139条查询结果,搜索用时 15 毫秒
91.
Hiroto MAEDA Kazuyuki SOGAWA Kazuko SAKAGUCHI Saori ABE Wataru SAGIZAKA Shunsuke MOCHIZUKI Waka HORIE Toshifumi WATANABE Yui SHIBATA Mamoru SATOH Akihiro SANDA Fumio NOMURA Jun SUZUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):937-943
Feline renal diseases are increasingly noted in veterinary practice. It is important to diagnose and identify the pathological basis of renal dysfunction accurately at an early stage, but there are only a few reports on this area in clinical veterinary medicine. We investigated the efficacy of measurement of urinary albumin (u-Alb) and urinary transferrin (u-Tf) for early diagnosis using 5-µl urine samples collected noninvasively by catheterization from normal (IRIS stage I) cats and cats with stage I chronic kidney disease (CKD). The u-Alb levels in normal and stage I CKD cats were 6.0 ± 4.5 and 11.2 ± 8.4 mg/dl, respectively, and the u-Tf levels were 0.09 ± 0.42 and 0.52 ± 0.79 mg/dl, respectively. Based on ROC curve analysis, the sensitivity and specificity of u-Alb and u-Tf were higher than those of the currently used biomarker, the plasma creatinine level. The sensitivity of u-Alb was higher than that of u-Tf, whereas
the specificity of u-Tf was higher than that of u-Alb. The validity of the threshold albumin level (20 mg/dl) was confirmed by measurements using SDS-PAGE. Since leakage of u-Tf in urine precedes leakage of u-Alb, inclusion of u-Tf in biochemistry tests may be appropriate for IRIS staging as a diagnostic marker of early diagnosis of renal disorder in cats. 相似文献
92.
A carbon-chromite fraction from the Allende C3V chondrite shows strikingly large isotopic enrichments of neodymium-142 (0.47 percent) and neodymium- 143 (36 percent). Both apparently formed by alpha decay of samarium-146 and samarium-147 (half-lives 1.03 x 10(8) and 1.06 x 10(11) years), but the isotopic enrichment was greatly magnified by recoil of residual nuclei into a carbon film surrounding the samarium-bearing grains. These data provide an improved estimate of the original abundance of extinct samarium-146 in the early solar system [(146)Sm/(144)Sm = (4.5 +/- 0.5) x 10(-3)], higher than predicted by some models of pprocess nucleosynthesis. It may be possible to use this isotopic pair as a chronometer of the early solar system. 相似文献
93.
Shimamura T Takamori A Ukeda H Nagata S Sawamura M 《Journal of agricultural and food chemistry》2000,48(4):1204-1209
Dihydropyrazine derivatives formed by the self-condensation reaction of D-glucosamine have the DNA breaking activity. To establish the monitoring method of the biological active dihydropyrazines, we investigated the relationship between the XTT (3'-[1-[(phenylamino)-carbonyl]-3, 4-tetrazolium]bis(4-methoxy-6-nitro)benzensulfonic acid hydrate) reducibility and the DNA breaking activity of aminosugars. Aminosugar in 50 mM sodium phosphate buffer (pH 7.4) was incubated at 37 degrees C. At a given time, the XTT reducibility and the DNA breaking activity of the incubated aminosugar were measured. Both XTT reducibility and DNA breaking activity showed a maximum value within 1-4 h after the incubation and then gradually decreased with the incubation time. Superoxide anion was suggested to involve in both of the DNA breaking activity and the XTT reducibility by the addition of the radical scavengers into those assay mixtures. The quantity of remaining covalently closed circular DNA and the XTT reducibility of all aminosugars showed a good correlation (r = 0.825, n = 26). This means that the XTT assay is applicable for the monitoring of those biologically active products derived from aminosugars when the participation of superoxide anion in DNA scission is recognized. 相似文献
94.
95.
Jian Gao Shunsuke Koshio Manabu Ishikawa Saichiro Yokoyama Daisuke Nose Tongjun Ren 《Journal of the World Aquaculture Society》2013,44(4):536-546
A feeding trial was conducted to determine the interactive effects of vitamin C (ascorbic acid, AsA) and E (α‐tocopherol, α‐Toc) supplementation with dietary oxidized fish oil (OFO) on the growth performance, whole‐body AsA, and α‐Toc concentrations and fatty acid composition of juvenile sea cucumber. In a 9‐wk feeding trial, juveniles (average weight: 0.6 ± 0.1 g) were cultured in twenty‐four 50‐L tanks (30 juveniles per tank) in triplicate, and fed with eight test diets containing two levels of OFO (8.9 and 156.9 meq/kg) with varying levels of vitamin C (500 and 1000 mg AsA equivalents/kg diet) and E (100 and 200 mg α‐Toc equivalents/kg diet) supplementation, respectively. Body weight gain of sea cucumber was significantly reduced by dietary OFO, while mortality and whole‐body thiobarbituric acid‐reactive substances value were increased significantly. Increasing dietary vitamin C and E levels significantly increased whole‐body α‐Toc and AsA concentrations, respectively. A high level of vitamin E combined with OFO led to consumption of AsA. Even with supplementation of a large dose of vitamin C and/or E in diets, growth performance could not be improved, probably due to the high levels of vitamins in the control diets. 相似文献
96.
Shunsuke Koshio Ron K. O'Dor John D. Castell 《Journal of the World Aquaculture Society》1990,21(3):160-169
When fed formulated diets containing 45% protein derived from the rock crab Cancer irroratus , wheat gluten, gelatin, lipids adjusted from 3–14% and carbohydrates adjusted from 5–24%, the average survival of bilaterally eyestalk ablated juvenile lobsters and intact controls were 91% and 99%, respectively over a 90-day experiment. Photopenad (13 light: 11 dark vs 3 light: 21 dark) did not have a significant effect on growth or survival of ablated or intact lobsters. The optimal digestible energy levels and protein/energy ratio for the greatest growth in both ablated and intact lobsters were 3.7 to 3.8 (kcal/g dry) and 0.12 [g protein/(kcal/g)], respectively. The best feed conversion ratios (0.97 and 1.40 diet given/weight gain) for ablated and intact lobsters were obtained with diets containing 3.83 kcal/g and 3.63 kcal/g diet, respectively. The present study also demonstrated that crab protein served as a good protein source for intact and ablated lobsters. 相似文献
97.
Tanaka E Okumura S Takamiya R Hosaka H Shimamura Y Murata M 《Journal of agricultural and food chemistry》2011,59(12):6705-6709
Cinnamaldehyde treatment inhibited the browning of cut lettuce during cold storage. In this study, to clarify the mechanism of inhibitory action of cinnamaldehyde against the browning and to show its microbiological merit, its effect on the browning of cut lettuce was compared to that of mild heat treatment. Both cinnamaldehyde and mild heat treatments inhibited the induction of phenylalanine ammonia-lyase (PAL) activity because of cutting. As a result, the biosynthesis of polyphenols, which are substrates of polyphenol oxidase, was inhibited. This reduction of polyphenol synthesis caused the inhibition of the browning. Cinnamaldehyde treatment repressed the induction of PAL mRNA, while mild heat treatment did not repress its induction. The increase in microbes in cut lettuce treated with cinnamaldehyde was less than that treated with mild heat after 12 days. 相似文献
98.
Growth and Survival of Larval Red Sea Bream Pagrus major and Japanese Flounder Paralichthys olivaceus Fed Microbound Diets 总被引:3,自引:1,他引:3
Akio Kanazawa Shunsuke Koshio Shin-Ichi Teshima 《Journal of the World Aquaculture Society》1989,20(2):31-37
To establish the practical use of microbound diets (MBD) for larval fish in mass seedling production, rearing experiments of larval red sea bream, Pagrus major , and Japanese flounder, Paralichthys olivaeeus , were conducted. A mixture of various protein sources was used, and dietary amino acid patterns were approximated to those of larval whole body protein. Two thousand red sea bream larvae and 1,000 Japanese flounder larvae, all 10 days old, were placed in 100 liter tanks with running sea water under ambient water temperature, which ranged from 18 to 20C. The particle size of MBD was 125 μm at the beginning of the experiment and adjusted as fish size increased thereafter. Employing MBD together with a small amount of live food could sustain the growth and survival of larval red sea bream and Japanese flounder. Thus, data from the present study indicates that substitution of artificial feeds for live foods is possible for larval fish production, although improvements in MBD diets may be necessary before they are adequate for large scale seedling production. 相似文献
99.
Md. Shah Alam Shin-ichi Teshima Manabu Ishikawa Daisuke Hasegawa & Shunsuke Koshio 《Aquaculture Research》2004,35(9):842-849
The requirements for essential amino acids by kuruma shrimp, Marsupenaeus japonicus (Bate) have not yet been determined using the dose–response method due to poor utilization of crystalline amino acids (CAA). In a previous study, we produced coated CAA that could be used to retard leaching losses. Therefore, to determine the arginine requirement of juvenile kuruma shrimp, a growth study was conducted using test diets containing six levels of coated arginine (from 1.20% to 3.19% of diet) for 42 days. Crystalline amino acids was supplemented to correspond to the amino acid pattern in the whole body protein of the kuruma shrimp except for arginine. To reduce leaching losses, CAA were coated with carboxymethylcellulose (CMC) and diets were further bound with CMC and k‐carrageenan. Test diets were fed to triplicate groups of juveniles (0.25±0.02 g) twice a day. Proximate composition and the amino acid composition of shrimp whole bodies were analysed at the end of the feeding trial. Percent body weight gain (WG), specific growth rate, feed intake, feed efficiency ratio and protein retention efficiency were significantly affected by the dietary arginine concentrations. Estimating the arginine requirement of kuruma shrimp using broken‐line analysis of percent WG resulted in a value of 2.66% of diet or 5.32% of protein. After correcting for leaching of arginine from the diets, the optimum dietary value of arginine for kuruma shrimp was a little lower than the estimated value. 相似文献